Facilities

Institute for Immunology and Informatics (iCubed)CoresRI badge

The Institute for Immunology and Informatics, along with the URI Biotechnology Center, occupies approximately 7,200 square feet of laboratory space, located at the Feinstein Providence Campus.

iCubed Labs

Laboratory of Viral Immunity and Pathogenesis (LVIP)

The LVIP is a biosafety level 2 laboratory focused on the study of host-virus interactions, with an emphasis on emerging and neglected infectious diseases and global health.

URI Biotechnology Manufacturing/Cytopathology Labs

  • Protein Purification Lab
  • Industry Training and Research Lab
  • Tissue Culture Lab
  • Cytopathology Research and Teaching Lab

Vaccine and Immunotherapeutic Research Laboratory (VIRL)

The Vaccine and Immunotherapeutic Research Laboratory is a mixed-use space consisting of a 1,200-square-foot wet lab and a 400-square-foot computer/bioinformatics suite.

Cell Analysis and Sorting Core Lab

This Core provides flow cytometry services using either a 4-laser, 12-parameter LSR II analyzer or an automated 3-laser, 8-color MACSQuant-10 analyzer, and automated cell sorting services using the AutoMACS platform housed in a class IIA biosafety cabinet.

iCubed Equipment

Cell Culture

  • Class IIA biosafety cabinets (5)
  • CO2incubators (5; one equipped with refrigeration for 28°C culture)
  • Refrigerated centrifuges (2)
  • Inverted microscope
  • Nexcelom Cellometer automated cell counters (2- T4, Mini)

Flow Cytometry and Immunoassays

  • BD LSRII flow cytometer with 4 lasers
  • Miltenyi MACSQuant-10 flow cytometer
  • CTL Analyzer for cell spot imaging and capture
  • Envision multilabel plate reader (includes filters for fluorescence, luminescence, and AlphaScreen)

Cryostorage

  • Liquid nitrogen storage tanks (7; combined capacity >100,000 vials)
  • Ultralow temperature freezers (5; combined capacity >100,000 vials)

Microscopy

  • EVOSfl fluorescence microscope (includes GFP, RFP, DAPI, and Cy5 cubes, 100× oil immersion objective)
  • BioTek Cytation3 cell imaging plate reader with CO2incubation chamber
  • Confocal microscope

Molecular Biology

  • Bacterial incubator
  • Eppendorf 5810R high speed centrifuge
  • Lab Companion shaking incubator
  • Electrophoresis equipment
  • Nanodrop spectrophotometer
  • Bio-Rad UVM spectrophotometer
  • AKTA Avant 25 FPLC
  • Thermo Fisher Speed Vac Evaporator
  • PCR hood
  • QIAcube (automated nucleic acid isolation)
  • QIAgility (liquid handler/automated PCR assay setup)
  • Bio-Rad dual 48-well PCR machine (2)
  • Bio-Rad CFX96 real-time PCR machine
  • Bio-Rad Chemi-Doc XRS imager

Other

  • MakerBot 3-D printer
  • Miltenyi AutoMacs cell isolation unit
  • Beckman XL100K ultracentrifuge
  • Dolomite microfluidics droplet generator with high speed camera
  • Agilent 2100 Bioanalyzer
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