CMI Core Lab
Facilities and Equipment
The CMI Core Main Lab occupies 650 sq. ft at the URI Providence Feisntein Campus. The core is responsible for providing 5 main services to TRIAD researchers.
This is accomplished using a BD LSRII flow cytometer with a 3 laser package allowing for 9 color analysis. The BD LSRII also supports 96 well and 384 well assay formats, as well as individual tubes utilizing the BD High Throughput System The current optics package on the BD LSR II is made up of 3 lasers:
- 488 nm Blue Laser supporting FSC, SSC, FITC, PE (Pe Cy-7), PerCP (PerCP Cy-5.5, PerCP Cy7)
- 633 nm Red Laser supporting APC, APC Cy-7 (APC h-7)
- 405 nm Violet Laser supporting Pacific Blue, AmCyan
- Other custom fluorphores are supported such as alexa fluor stains, please inquire if you have any questions as to fluorophore selection.
ELISpot Assay Support
This is accomplished using a CTL Ltd. S5 UV Analyzer which is comprised of a precision microscope and digital camera package that can scan and capture images of individual ELISpot wells and Immunospot 5.0 which is a software package capable of automatically reading and analyzing spot size, density and intensity.
Cryogenic Storage The core provides the ability to stably freeze cell and tissue culture samples at a controlled rate optimized for cell recovery using a Thermo Cryomed 7450 Controlled Rate Freezer, and maintains long term ultra low temp storage using a Thermo Cryoplus 7400 LN2 Dewar which has the capability iof storing up to 6800 2 ml vials.
The preparation of liposome encased peptides, proteins or DNA constructs is becoming available in conjunction with Dr. Geoff Bothun of URI’s Chemical Engineering Department in Kingston. Sample preparation in Providence is performed using a Buchi R114 Rotovap, a Misonix 2000 sonicator and a Labconco Freezone 1 L Lyopholizer. Characterization is currently being perfoemd in the Bothun Lab in Kingston.
Transgenic Mouse Colony Maintenance
The core maintains 3 human transgenic mouse colonies currently at Taconic Farms in upstate New York. We are currently maintaining A2DR1, DR3 and DR4 mouse lines.
BSLII Cell and Tissue Culture Lab
The CMI Core BSLII lab occupies 200 sq. ft of area within the CMI Core Main Lab and is used as a central lab for all cell and tissue culture performed by the TRIAD program at URI’s Providence Campus. It supports assays performed in the Main Lab, (ie. Flow cytometry, ELISpot and in preparation of samples for cryogenic storage).
Equipment within the lab consists of the following:
- NuAir 6ft Laminar Flow Hood
- Labconco 4ft Laminar Flow Hood
- Thermo Stericult CO2 Incubator
- Fisher Isotemp CO2 Incubator
- Thermo Heraues Multifuge X3R
- Nexcelom Auto T4 Cell Counter
- Zeiss Inverted Microscope
- Thermo Isotemp Waterbath
BSLII Animal Facility
Located at Peckham Farm on the University of Rhode Island’s Kingston campus, the BSLII animal facility is a 400 sq, ft lab equipped with state of the art Optimice Animal Cages, 2 Biosafety cabinets, CO2 Incubators, refrigeration, ultra low temp freezer and autoclave. It can be made use of by contacting Joe Desrosiers at CMICore.URI@gmail.com