On Friday August 12 iCubed will welcome Dr. Anne Hosmalin of the Cochin Institute at Paris-Descartes University in France to deliver a lecture titled “Dendritic cells and HIV infection.” Dr. Hosmalin is a dendritic cell expert and was instrumental in the laboratory immunology training of iCubed’s own Dr. Annie De Groot.

The seminar will take place at the University of Rhode Island’s Feinstein Providence Campus in room 334C beginning at 12:30pm. Following the seminar, a discussion session will take place over lunch in the iCubed offices of room 302F. We encourage everyone to take this opportunity to meet a true expert in her field.

Dr. Hosmalin’s lecture abstract, bio, as well as two sample publications are listed below.

Dendritic cells and HIV infection

Anne Hosmalin, M.D., Ph.D.

Research Director, Professor

Cochin Institute, INSERM U1016, CNRS UMR 8104, Paris-Descartes University, France

Dendritic cells play a dual role in HIV infection, acting as antiviral effectors as well as portals of entry and actorsin pathogenesis. Their numbers and functions are reduced in the circulation of HIV patients. Their specialized crosspresentation function must be exploited for vaccine design.

Biography

Anne Hosmalin graduated as a M.D. from Paris-Descartes University in 1986 and as a Ph.D. in Immunology from Paris-Pierre & MarieCurie University in 1990. She was a post-doctoral fellow at the NCI, NIH,Bethesda, MD, USA with Dr. J.A. Berzofsky. Since 1998, she is the head of the“Antigen Presentation by Dendritic Cell” Team at the Cochin Institute (U INSERM1016, UMR CNRS 8104, Paris-Descartes University) in Paris. She is a Research Director at the National Center for Scientific Research (CNRS). She is the head of the Coordinate Action AC31 at the National Agency for AIDS Research (ANRS)and the current President of the Scientific Specialist Committee CSS5 on Immunology, Hematology and Infectious Disease at the National Medical ResearchInstitute (INSERM). She has been working on CD8 T cell responses against HIV,synthetic vaccines, antigen presentation by dendritic cells and on the role of dendritic cells in HIV infection.

5 most cited publications out of 76 international journals and books

– Takahashi, H., J. Cohen, A. Hosmalin, … R.N.Germain, and J.A. Berzofsky. 1988. An immunodominant epitope of the HIVenvelope gp160 recognized by class I MHC molecule-restricted murine cytotoxic Tlymphocytes. PNAS 85, 3105

– Pacanowski, J*., Kahi, S*., Baillet, M.,Lebon, P., Deveau, C., Goujard, C., Meyer, L., Oksenhendler, E., Sinet, M., andHosmalin, A. Reduced blood CD123+ and CD11c+ dendritic cell numbers in primaryHIV-1 infection. Blood, 2001; 98:3016-3021.

– Seifert, U., … Schild, H., Haas, G., Kloetzel,P.M., Reiss, Y.  and Hosmalin, A. An essential role for Tripeptidylpeptidase  (TPPII) in the generation of MHC class I epitopes . NatureImmunol, 2003; 4:375

– Marañón, C., Desoutter, J.F., Hoeffel, G.,Cohen, W., Hanau, D., and Hosmalin, A. DC cross-present HIV antigens from liveas well as apoptotic infected CD4+T lymphocytes. PNAS, 2004;101:6092

– Hoeffel, G., … Hosmalin, A. and Marañón, C.Antigen cross-presentation by human  Plasmacytoid Dendritic Cells.Immunity, 2007 ; 27 :481

-Crozat K,Feuillet V*, … Dutertre CA*, … Hosmalin A, Dalod M. The XC chemokine receptor 1is a conserved selective marker of mammalian cells homologous to mouseCD8alpha+ dendritic cells. J Exp Med. 2010 Jun 7;207(6):1283-92. (2010)

Publication: (1)

Blood. 2010 Jun 3;115(22):4412-20. Epub  2010 Mar 22.

Cross-presentation by dendritic cells from live cells induces protective immune responses in vivo

Matheoud D, Perié L, Hoeffel G, Vimeux L, Parent I, Marañón C, Bourdoncle P, Renia L, Prevost-Blondel A, Lucas B, Feuillet V, Hosmalin A.

Source

Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique (Unite Mixte de Recherche 8104), Paris, France.

Abstract

Cross-presentation is an essential mechanism that allows dendritic cells (DCs) to efficiently present exogenous antigens to CD8(+) T cells. Among cellular antigen sources, apoptotic cells are commonly considered as the best for cross-presentation by DCs. However, the potential of live cells as a source of antigen has been overlooked. Here we explored whether DCs were able to capture and cross-present antigens from live cells. DCs internalized cytosolic and membrane material into vesicles from metabolically labeled live cells. Using time-lapse confocal microscopy in whole spleens, we showed that DCs internalized material from live cells in vivo. After ovalbumin uptake from live cells, DCs cross-primed ovalbumin-specific naive OT-I CD8(+) T cells in vitro. Injected into mice previously transferred with naive OT-I T cells, they also cross-primed in vivo, even in the absence of endogenous DCs able to present the epitope in the recipient mice. Interestingly, DCs induced stronger natural CD8(+) T-cell responses and protection against a lethal tumor challenge after capture of antigens from live melanoma cells than from apoptotic melanoma cells. The potential for cross-presentation from live cells uncovers a new type of cellular intercommunication and must be taken into account for induction of tolerance or immunity against self, tumors, grafts, or pathogens.

Publication: (2)

Vaccine. 2010 Aug 2;28(34):5582-90. Epub  2010 Jun 25.

Sublingual immunization with an HIV subunit vaccine induces antibodies and cytotoxic T cells in the mouse female genital tract

Hervouet C, Luci C, Cuburu N, Cremel M, Bekri S, Vimeux L, Marañon C, Czerkinsky C, Hosmalin A, Anjuère F.

Source

Inserm, U634, Faculté de Médecine Pasteur, 06107 Nice cedex 2, France.

Abstract

A vaccine against heterosexual transmission by human immunodeficiency virus (HIV) should generate cytotoxic and antibody responses in the female genital tract and in extra-genital organs. We report that sublingual immunization with HIV-1 gp41 and a reverse transcriptase polypeptide coupled to the cholera toxin B subunit (CTB) induced gp41-specific IgA antibodies and antibody-secreting cells, as well as reverse transcriptase-specific CD8 T cells in the genital mucosa, contrary to intradermal immunization. Conjugation of the reverse transcriptase peptide to CTB favored its cross-presentation by human dendritic cells to a T cell line from an HIV(+) patient. Sublingual vaccination could represent a promising vaccine strategy against heterosexual transmission of HIV-1.